私たちの研究室では遺伝子発現における翻訳のしくみやncRNAの機能などについて研究しています

研究業績

学術論文・著書

2014

Himeno, H., Kurita, D. & Muto, A. (2014)  Mechanism of trans-translation revealed by in vitro studies. Frontiers Microbiol. 5 65.

Himeno, H., Kurita, D. & Muto, A. (2014) tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell. Frontiers Genet. 5 66.

Yang, Z., Guo, Q., Goto, S., Chen, Y., Li, N., Yan, K., Zhang, Y., Muto, A., Deng, H., Himeno, H., Lei, J. & Gao, N. (2014) Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence. Protein Cell 5 394-407.

Kurita, D., Miller, M., Muto, A., Buskirk, A. & Himeno, H.  (2014) Rejection of tmRNA• SmpB after GTP hydrolysis by EF-Tu on ribosomes stalled on intact mRNA. RNA 20 1706-1714.

Kurita, D., Chadani, Y., Muto, A., Abo, T. & Himeno, H. (2014) ArfA recognizes the lack of mRNA in the mRNA channel after RF2 binding for ribosome rescue. Nucleic Acids Res. 42 13339-13352.

Himeno, H., Nameki, N., Kurita, D., Muto, A. & Abo T.: Ribosome rescue systems in bacteria. Biochimie, in press.

 

2013

Guo, Q., Goto, S., Chen, Y., Feng, B., Xu, Y., Muto, A., Himeno, H., Deng, H., Lei, J. & Gao, N. (2013) Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process. Nucleic Acids Res. 41 2609-2620.

Goto, S., Muto, A. & Himeno, H. (2013) GTPases involved in bacterial ribosome maturation. J. Biochem. 153 403-414.

Hase, Y., Tarusawa, T., Muto, A. & Himeno, H. (2013) Impairment of ribosome maturation or function confers salt resistance on Escherichia coli cells. PLoS ONE, 8(5) e65747.

姫野俵太、栗田大輔、武藤昱:「tmRNA:2つの機能を有するRNA分子」、実験医学、54-60、羊土社(2013)

 

2012

Kurita, D., Muto, A. & Himeno, H. (2012) In vitro trans-translation assay. Methods Mol. Biol.,  Vol. 905 (Bacterial Regulatory RNA) 311-325, Humana Press.

姫野俵太:「2つのtRNA/mRNAハイブリッドによるトランストランスレーション」、Viva Origino, 40(4) (2012)

姫野俵太:「弘前で咲いたRNAの研究」、弘前大学知の散歩道、147-155、弘前大学出版会(2012)

 

2011

Kurita, D., Muto, A. & Himeno, H. (2011) tRNA/mRNA mimicry by tmRNA and SmpB in trans-translation. J. Nucleic Acids 2011 Article ID 130581, doi:10.4061/2011/130581.

Goto, S., Kato, S., Kimura, T., Muto, A. & Himeno, H. (2011) RsgA releases RbfA from 30S ribosomal subunit during a late stage of ribosome biosynthesis. EMBO J. 30 104-114.

Himeno, H., Kurita, D. & Muto, A.: Trans-translation by tmRNA and SmpB: a bacterial quality control system of translation. In Kevin V. Urabano Ed., Advances in Genetics Research – Vol. 6, 161-203, (Chapter 3), Nova Science Publishers Inc. (2011).

姫野俵太:The 3rd International Symposium on Protein Community 「タンパク質の社会」領域主催国際会議(ISPC-NARA2010)パートI、Protein Community, Vol. 7, 20-22 (2011)

渡辺公綱、姫野俵太:生命化学II 第2版 −遺伝子の働きとその応用−、丸善 (2011)

 

2010

Himeno, H. (2010) Novel factor rescues ribosomes trapped on non-stop mRNAs. Mol. Microbiol. 78 789-791.

Hokii, Y., Sasano, Y., Sato, M., Sakamoto, H., Sakata, K., Shingai, R., Taneda, A., Oka, S., Himeno, H., Muto, A., Fujiwara, T. & Ushida, C. (2010) A small nucleolar RNA functions in rRNA processing in C. elegans. Nucleic Acids Res. 38 5909-5918.

Kurita, D., Muto, A. & Himeno, H. (2010) Role of the C-terminal tail of SmpB in the early stage of trans-translation. RNA 16 980-990.

姫野俵太、栗田大輔、武藤昱:「2つのtRNA/mRNAハイブリッド –トランス・トランスレーション-」、mRNAプログラム 多様性と非対称性の獲得戦略 (稲田利文、大野睦人編)、2201-2206、共立出版 (2010)

 

2009

Hase, Y., Yokoyama, S., Muto, A. & Himeno, H. (2009) Removal of a ribosome small subunit-dependent GTPase confers salt-resistance on Escherichia coli cells. RNA 15 1766-1774.

Ujiie, H., Matsutani, T., Tomatsu, H., Fujihara, A., Ushida, C., Miwa, Y., Fujita, Y., Himeno, H. & Muto, A. (2009) Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis. J. Biochem. 145 59-66.

Himeno, H., Kurita, D. & Muto, A. (2009) Trans-translation by tmRNA and a protein mimicking tRNA and mRNA. In Toma E. Esterhouse & Lado B. Petrinos Eds., Protein Biosynthesis, pp69-107, Nova Science Publishers Inc.

姫野俵太、栗田大輔、武藤昱:「2つのtRNA/mRNAハイブリッド –トランス・トランスレーション-」、蛋白質核酸酵素増刊、54 (16)、2201-2206、共立出版 (2009)

姫野俵太:医学書院医学大辞典第2版(伊藤正男・井村裕夫・高久史麿編)、医学書院(2009)

渡辺公綱、鈴木勉、姫野俵太:「翻訳」、改訂第3版分子生物学イラストレイテッド(田村隆明、山本雅編)、48-56、羊土社(2009)

 

2008

Abe, T., Sakaki, K., Fujihara, A., Ujiie, H., Ushida, C., Himeno, H., Sato, T. & Muto, A. (2008) tmRNA-dependent trans-translation is required for sporulation in Bacillus subtilis. Mol. Microbiol. 69 1491-1498.

Kimura, T., Takagi, K., Hirata, Y., Hase, Y., Muto, A. & Himeno, H. (2008) Ribosome-small-subunit-dependent GTPase interacts with the tRNA binding sites on the ribosome. J. Mol. Biol. 381 467-477.

姫野俵太、武藤昱:「トランストランスレーション」、キーワード:蛋白質の一生(遠藤斗志也、小椋光、永田和宏、森和俊、田口英樹、吉田賢右編)、975、共立出版 (2008)

姫野俵太:「窒素同化とアミノ酸代謝」、ベーシックマスター生化学(大山隆監修、西川一八、清水光弘編)、オーム社、249-265 (2008)

栗田大輔、武藤昱、姫野俵太:「mRNAを擬態するタンパク質」、化学と生物、46(7)、465-471、学会出版センター(2008)

姫野俵太、武藤昱:「トランストランスレーション」、蛋白質核酸酵素、53(8)、975、共立出版 (2008)

 

2007

Kurita, D., Sasaki, R., Muto, A. & Himeno, H. (2007) Interaction of SmpB with ribosome from directed hydroxyl radical probing. Nucleic Acids Res. 35 7248-7255.

Konno, T., Kurita, D., Takada, K., Muto, A. & Himeno, H. (2007) A functional interaction of SmpB with tmRNA for determination of the resuming point of trans-translation. RNA 13 1723-1731.

Takada, K., Takemoto, C., Kawazoe, M., Konno, T., Hanawa-Suetsugu, K., Lee, S., Shirouzu, M., Yokoyama, S., Muto, A. & Himeno, H. (2007) In vitro trans-translation of Thermus thermophilus: Ribosomal protein S1 is not required for the early stage of trans-translation. RNA 13 503-510.

姫野俵太、栗田大輔、高田一馬、今野貴之、塙(末次)京子、竹本千重、川添将仁、横山茂之、行木信一、河合剛太、武藤昱:「トランス・トランスレーションの分子メカニズム」、生化学、79, 213-221 (2007)

 

2006

Hashimoto, M., Murakami, T., Funahashi, K., Tokunaga, T., Nihei, K., Okuno, T., Kimura, T. & Himeno, H. (2006) An RNA polymerase inhibitor, cyclothiazomycin B1, and its isomer. Bioorg. Med. Chem. 14 8259-8270.

姫野俵太、武藤昱:「トランストランスレーション」、蛋白質核酸酵素、51(15), 2321-2327、共立出版(2006)

 

2005

Nameki, N., Someya, T., Okano, S., Suemasa, R., Kimoto, M., Hanawa-Suetsugu, K., Terada, T., Shirouzu, M., Hirao, I., Takaku, H., Himeno, H., Muto, A., Kuramitsu, S., Yokoyama, S. & Kawai, G. (2005) Interaction analysis between tmRNA and SmpB from Thermus thermophilus. J. Biochem. 138 729-739.

Asano, K., Kurita, D., Takada, K., Konno, T., Muto, A. & Himeno, H. (2005) Competition between trans-translation and termination or elongation of translation. Nucleic Acids Res. 33 5544-5552.

姫野俵太:「タンパク質の合成」、タンパク質科学イラストレイテッド(竹縄忠臣編)、34-44、羊土社 (2005)

姫野俵太、武藤昱:「翻訳と共役した蛋白質品質管理システム:トランストランスレーション」、細胞における蛋白質の一生生成・成熟・輸送・管理・分解・病態(小椋光、遠藤斗志也、森正敬、吉田賢右編)、1062-1066、共立出版(2005)

 

2004

Konno, T., Takahashi, T., Kurita, D., Muto, A. & Himeno, H. (2004) A minimum structure of aminoglycosides that causes an initiation shift of trans-translation. Nucleic Acids Res. 32 4119-4126.

Himeno, H., Hanawa-Suetsugu, K., Kimura, T., Takagi, K., Sugiyama, W., Shirata, S., Mikami, T., Odagiri, F., Osanai, Y., Watanabe, D., Goto, S., Kalachnyuk, L., Ushida, C. & Muto, A. (2004) A novel GTPase activated by the small subunit of ribosome. Nucleic Acids Res. 32 5303-5309.

姫野俵太、武藤昱:「翻訳と共役した蛋白質品質管理システム:トランストランスレーション」、蛋白質核酸酵素、49(7)、1062-1066、共立出版(2004)

武藤昱、姫野俵太:「tmRNAによるトランス翻訳機構と生理的機能」、RNAの細胞生物学(中村義一、坂本博編)、346-356、共立出版(2004)

 

2003

Takahashi, T., Konno, T., Muto, A. & Himeno, H. (2003) Various effects of paromomycin on tmRNA-directed trans-translation. J. Biol. Chem. 278 27672-27680.

姫野俵太:「タンパク質合成」、わかる実験医学シリーズ タンパク質がわかる(竹縄忠臣編)、26-35、羊土社(2003)

渡辺公綱、鈴木勉、姫野俵太:「翻訳」、改訂第2版分子生物学イラストレイテッド(田村隆明、山本雅編)、56-64、羊土社 (2003)

渡辺公綱、姫野俵太:生命化学II −遺伝子の働きとその応用−、丸善 (2003)

武藤昱、姫野俵太:「tmRNAによるトランス翻訳機構と生理的機能」、蛋白質核酸酵素、48(4)、346-356、共立出版(2003)

姫野俵太:医学書院医学大辞典(伊藤正男・井村裕夫・高久史麿編)、医学書院 (2003)

 

2002

Fujihara, A., Tomatsu, H., Inagaki, S., Tadaki, T., Ushida, C., Himeno, H. & Muto, A. (2002) Detection of tmRNA-mediated trans-translation products in Bacillus subtilis. Genes to Cells 7 343-350.

Hanawa-Suetsugu, K., Takagi, M., Inokuchi, H., Himeno, H. & Muto, A. (2002) SmpB functions in various steps of trans-translation. Nucleic Acids Res. 30 1620-1629.

Ito, K., Tadaki, T., Lee, S., Takada, K., Muto, A. & Himeno, H. (2002) Trans-translation mediated by Bacillus subtilis tmRNA. FEBS Lett. 516 245-252.

姫野俵太、武藤昱:「枯草菌Bacillus subtilisのtmRNAによるトランス・トランスレーション」、ゲノムニュース、No 4, 24-25、クバプロ (2002)

 

2001

Sato, K., Sugita, T., Kobayashi, K., Fujita, K., Fujii, T., Matsumoto, Y., Mikami, T., Nishizuka, N., Nishizuka, S., Suda, M., Shojima, K., Takahashi, G., Himeno, H., Muto, A. & Ishida, S. (2001) Localization of mitochondrial ribosomal RNAs on the chromatoid bodies of the marine planarian polyclad embryos. Develop. Growth Differ. 43 107-114.

Lee, S., Ishii, M., Tadaki, T., Muto, A. & Himeno, H. (2001) Determinants on tmRNA for initiating efficient and precise trans-translation: Some mutations upstream of the tag-encoding sequence of Escherichia coli tmRNA shift the initiation point of trans-translation in vitro. RNA 7 999-1012.

Hanawa-Suetsugu, K., Bordeau, V., Himeno, H., Muto, A. & Felden, B. (2001) Importance of the conserved nucleotides around the tRNA-like structure of Escherichia coli transfer-messenger RNA for protein tagging. Nucleic Acids Res. 29 4663-4673.

 

2000

Nameki, N., Tadaki, T., Himeno, H. & Muto, A. (2000) Three of four pseudoknots in tmRNA are interchangeable and are substitutable with single-stranded RNAs. FEBS Lett. 470 345-349.

Muto, A., Fujihara, A., Ito, K., Matsuno, J., Ushida C. & Himeno, H. (2000) Requirement of transfer-messenger RNA (tmRNA) for the growth of Bacillus subtilis under stresses. Genes Cells 5 627-636.

武藤昱、姫野俵太:tmRNA、RNA研究の最前線、49-54、志村令郎、渡辺公綱編、スプリンガー・フェアラーク東京(2000)

牛田千里、姫野俵太、武藤昱:RNA interference、RNA研究の最前線、92-97、志村令郎、渡辺公綱編、スプリンガー・フェアラーク東京(2000)

姫野俵太、武藤昱:tRNAおよび低分子RNAの機能解析、遺伝子発現研究法(生物化学実験法43、121-141、江尻慎一郎、平秀晴、堤賢一、志村憲助編、学会出版センター (2000)

 

1999

Nameki, N., Felden, B., Atkins, J.F., Gesteland, R.F., Himeno, H. & Muto, A. (1999) Functional and structural analysis of a pseudoknot upstream of the tag-encoded sequence in E. coli tmRNA. J. Mol. Biol. 286 733-744.

Nameki, N., Tadaki, T., Muto, A. & Himeno, H. (1999) Amino acid acceptor identity switch of Escherichia coli tmRNA from alanine to histidine in vitro. J. Mol. Biol. 289 1-7.

Nameki, N., Chattopadhyay, P., Himeno, H., Muto, A. & Kawai, G. (1999) An NMR and mutational study of an RNA pseudoknot of E. coli tmRNA involved in trans-translation. Nucleic Acids Res. 27 3667-3675.

Soma, A., Uchiyama, K., Sakamoto, T., Maeda, M. & Himeno, H. (1999) Unique recognition style of tRNALeu by Haloferax volcanii leucyl-tRNA synthetase. J. Mol. Biol. 293 1029-1038.

姫野俵太:tmRNAによるtrans-translationに関する研究、生化学、79, 1119-1130 (1999).

 

1998

Muto, A., Ushida, C. & Himeno, H. (1998) A bacterial RNA that functions as both a tRNA and an mRNA. Trends Biochem. Sci. 23 25-29.

Felden, B., Hanawa, K., Atkins, J.F., Himeno, H., Muto, A., Gesteland, R.F., McCloskey, J.A. & Crain, P.F. (1998) Presence and location of modified nucleotides in E. coli tmRNA. Structural mimicry with tRNA acceptor branches. EMBO J. 17 3188-3196.

Soma, A. & Himeno, H. (1998) Cross-species aminoacylation of tRNA with a long variable arm between Escherichia coli and Saccharomyces cerevisiae. Nucleic Acids Res. 26 4374-4381.

Morita, E., Ebina, H., Muto, A., Himeno, H., Hatakeyama, K. & Sugiyama, K. (1998) Primary structures of hemagglutinin-esterase and spike glycoproteins of murine coronavirus DVIM. Virus Genes 17 123-128.

渡辺公綱、井上暁夫、姫野俵太:翻訳、61-69、分子生物学イラストレイテッド(田村隆明、山本雅編)、羊土社 (1998)

武藤昱、牛田千里、姫野俵太:バクテリアで見つかったtRNAとmRNAの両方の働きをもつRNA、蛋白質核酸酵素、43, 1433-1442、共立出版 (1998)

 

1997

Felden, B., Himeno, H., Muto, A., McCutcheon, J., Atkins, J.F. & Gesteland, R.F. (1997) Probing the structure of the E. coli 10Sa RNA (tmRNA). RNA 3 89-103.

Himeno, H., Sato, M., Tadaki, T., Fukushima, M., Ushida, C. & Muto, A. (1997) In vitro trans translation mediated by alanine-charged 10Sa RNA. J. Mol. Biol. 268 803-808.

Himeno, H., Yoshida, S., Soma, A. & Nishikawa, K. (1997) Only one nucleotide insertion to the long variable arm confers an efficient serine acceptor activity upon Saccharomyces cerevisiae tRNALeu in vitro. J. Mol. Biol. 268 704-711.

姫野俵太、牛田千里、武藤昱:tmRNA: tRNAとmRNAの両方の機能を持つRNA、生化学、69、1204-1208 (1997)

牛田千里、姫野俵太、武藤昱:第2回The RNA Societyミーティング、蛋白質核酸酵素、42、2571-2573、共立出版 (1997)

 

1996

Soma, A., Kumagai, R., Nishikawa, K. & Himeno, H. (1996) The anticodon loop is a major identity determinant of Saccharomyces cerevisiae tRNALeu. J. Mol. Biol. 263 707-714.

Muto, A., Sato, M., Tadaki, T., Fukushima, M., Ushida, C. & Himeno, H. (1996) Structure and function of bacterial 10Sa RNA: trans-translation system. Biochimie 78 985-991.

Felden, B., Himeno, H., Muto, A., Atkins, J.F. & Gesteland, R.F. (1996) Structural organization of E. coli tmRNA. Biochimie 78 979-983.

Tadaki, T., Fukushima, M., Ushida, C., Himeno, H. & Muto, A. (1996) Interaction of 10Sa RNA with ribosomes in Escherichia coli. FEBS Lett. 399 223-226.

 

1995

Nameki, N., Asahara, H., Shimizu, M., Okada, N. & Himeno, H. (1995) Identity elements of Saccharomyces cerevisiae tRNAHis. Nucleic Acids Res. 23 389-394.

Asakawa, S., Himeno, H., Miura, K. & Watanabe, K. (1995) Nucleotide sequence and gene organization of the starfish Asterina pectinifera mitochondrial genome. Genetics 140 1047-1060.

 

1994

Asahara, H., Himeno, H., Tamura, K., Nameki, N., Hasegawa, T. & Shimizu, M. (1994) Escherichia coli seryl-tRNA synthetase recognizes tRNASer by its characteristic tertiary structure. J. Mol. Biol. 236 738-748.

Ushida, C., Himeno, H., Watanabe, T. & Muto, A. (1994) tRNA-like structures in 10Sa RNAs of Mycoplasma capricolum and Bacillus subtilis. Nucleic Acids Res. 22 3392-3396.

Tamura, K., Nameki, N., Hasegawa, T., Shimizu, M. & Himeno, H. (1994) Role of the CCA terminal sequence of tRNAVal in aminoacylation with valyl-tRNA synthetase. J. Biol. Chem. 269 22173-22177.

清水幹夫、姫野俵太:tRNAアイデンティティ、生化学、66、320-341 (1994)

 

1993

Asahara, H., Himeno, H., Tamura, K., Hasegawa, T., Watanabe, K. & Shimizu, M. (1993) Recognition nucleotides of Escherichia coli tRNALeu and its elements facilitating discrimination from tRNASer and tRNATyr. J. Mol. Biol. 231 219-229.

姫野俵太:ベクターDNAの分離精製. 分離精製技術ハンドブック、日本化学会編、708-711、丸善(1993)

 

1992以前

Hasegawa, T., Miyano, M., Himeno, H., Sano, Y., Kimura, K. & Shimizu, M. (1992) Identity determinants of E. coli threonine tRNA. Biochem. Biophys. Res. Commun. 184 478-484.

Tamura, K., Himeno, H., Asahara, H., Hasegawa, T. & Shimizu, M. (1992) In vitro study of E. coli tRNAArg and tRNALys identity elements. Nucleic Acids Res. 20 2335-2339.

Shimizu, M., Asahara, H., Tamura, K., Hasegawa, T. & Himeno, H. (1992) The role of anticodon bases and the discriminator nucleotide in the recognition of some E. coli tRNAs by their aminoacyl-tRNA synthetases. J. Mol. Evol. 35 436-443.

Nameki, N., Tamura, K., Himeno, H., Hasegawa, T. & Shimizu, M. (1992) Escherichia coli tRNAAsp recognition mechanism differing from that of the yeast system. Biochem. Biophys. Res. Commun. 189 856-862.

Asahara, H., Himeno, H. & Shimizu, M. (1991) A recognition model of tRNASer by seryl-tRNA synthetase in E. coli. Chem. Lett. 363-366.

Kumazawa, Y., Himeno, H., Miura, K. & Watanabe, K. (1991) The unilateral aminoacylation specificity between bovine mitochondria and eubacteria. J. Biochem. 109 421-427.

Asakawa, S., Kumazawa, Y., Araki, T., Himeno, H., Miura, K. & Watanabe, K. (1991) Strand-specific nucleotide composition bias in echinoderm and vertebrate mitochondrial genomes. J. Mol. Evol. 32 511-520.

Tamura, K., Himeno, H., Asahara, H., Hasegawa, T. & Shimizu, M. (1991) Identity determinants of E. coli tRNAVal. Biochem. Biophys. Res. Commun. 177 619-623.

Tamura, K., Asahara, H., Himeno, H., Hasegawa, T. & Shimizu, M. (1991) Identity elements of E. coli tRNAAla. J. Mol. Recog. 4 129-132.

Himeno, H., Hasegawa, T., Asahara, H., Tamura, K. & Shimizu, M. (1991) Identity determinants of E. coli tryptophan tRNA. Nucleic Acids Res. 19 6379-6382.

Himeno, H., Hasegawa, T., Ueda, T., Watanabe, K. & Shimizu, M. (1990) Conversion of aminoacylation specificity from tRNATyr to tRNASer in vitro. Nucleic Acids Res. 18 6815-6819.

渡辺公綱、姫野俵太:RNA editing. 化学、化学同人、11 806-807 (1990).

Himeno, H., Hasegawa, T., Ueda, T., Watanabe, K., Miura, K. & Shimizu, M. (1989) Role of the extra G-C pair at the end of the acceptor stem of tRNAHis in aminoacylation. Nucleic Acids Res. 17 7855-7863.

Hasegawa, T., Himeno, H., Ishikura, H. & Shimizu, M. (1989) Discriminator base of tRNAAsp is involved in amino acid acceptor activity. Biochem. Biophys. Res. Commun. 163 1534-1538.

Himeno, H., Matsushima, H. & Sano, K. (1988) Scanning electron microscopic study on the in vitro organogenesis of saffron stigma- and style-like structures. Plant Science 58 93-101.

Himeno, H., Masaki, H., Kawai, T., Ohta, T., Kumagai, I., Miura, K. & Watanabe, K. (1987) Unusual genetic codes and a novel gene structure for tRNASerAGY in starfish mitochondrial DNA. Gene 56 219-230.

Sano, K. & Himeno, H. (1987) In vitro proliferation of saffron (Crocus sativus L.) stigma. Plant Cell Tissue Organ Culture 11 159-166.

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